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Results: Results showed significant improvements after 14 days supplementation in the first period, as well as after 28 days in the second administration period, compared to placebo

Quantification of steroidogenic acute regulatory gene expression RNA Isolation, cDNA synthesis, and real-time quantitative polymerase chain reaction The homogenate was directly suspended in TRIzol reagent, followed by the extraction of mRNA

Reactions that warrant attention include persistent swelling or redness lasting more than 24 hours, significant pain beyond the initial injection moment, the development of hard nodules that do not resolve within a day or two, fever or systemic symptoms following injection, or any sign of infection at the injection site including warmth, pus, or spreading redness

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